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Species Reactivity
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验证数据展示

  • WB analysis using 32731-1-AP

    WB analysis using 32731-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32731-1-AP (CD36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 32731-1-AP

    IHC staining of mouse spleen using 32731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32731-1-AP (CD36 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 32731-1-AP

    IHC staining of mouse spleen using 32731-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32731-1-AP (CD36 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD36 Polyclonal antibody

CD36 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32731-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

Glycoprotein IIIb, GPIIIB, GPIV, PAS IV, PAS-4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32731-1-AP targets CD36 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 CD36 fusion protein Eg3464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD36 antigen
别名 Glycoprotein IIIb, GPIIIB, GPIV, PAS IV, PAS-4
计算分子量53 kDa
观测分子量80-85 kDa
GenBank蛋白编号NM_007643.4
基因名称 Cd36
Gene ID (NCBI) 12491
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ08857
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD36, also named as GP3B and GP4, is a membrane glycoprotein present on platelets, monocytes, erythroid precursors, endothelial cells, and several tumor cell lines. CD36 binds to collagen, thrombospondin, anionic phospholipids, long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport and oxidized LDL. CD36 may function as a cell adhesion molecule. It mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Mutation of CD36 will cause platelet glycoprotein IV deficiency which known as CD36 deficiency. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7).

实验方案

Product Specific Protocols
WB protocol for CD36 antibody 32731-1-APDownload protocol
IHC protocol for CD36 antibody 32731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols