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验证数据展示

  • FC experiment of human PBMCs using CR-65254

    FC experiment of human PBMCs using CR-65254

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD35 (CR-65254, Clone:E11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CR-65254

    FC experiment of human PBMCs using CR-65254

    1X10^6 human PBMCs were surface stained with 5 ul Cardinal Red™ Anti-Human CD35 (CR-65254, Clone:E11) (red) or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated.

Cardinal Red™ Anti-Human CD35 (E11)

CD35 Monoclonal Antibody for FC
Cat No. CR-65254

产品说明书

CloneNo. E11

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

KN, E11, CR1, Complement receptor type 1, C3BR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Cardinal Red™
规格价格库存

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-65254 targets CD35 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

fusion protein

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3b/4b) receptor 1 (Knops blood group)
别名 KN, E11, CR1, Complement receptor type 1, C3BR
计算分子量 224 kDa
GenBank蛋白编号NM_000573
基因名称 CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_3084987
偶联类型 Cardinal Red™ Fluorescent Dye
最大激发/发射波长592 nm / 611 nm
形式Liquid
纯化方式N/A
UNIPROT IDP17927
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ CD35 antibody CR-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols