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验证数据展示

  • WB analysis of THP-1 using 84341-3-RR (same clone as 84341-3-PBS)

    WB analysis of THP-1 using 84341-3-RR (same clone as 84341-3-PBS)

    WB result of CD33 antibody (84341-3-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD33 transfected THP-1 cells. This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

  • WB analysis using 84341-3-RR (same clone as 84341-3-PBS)

    WB analysis using 84341-3-RR (same clone as 84341-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84341-3-RR (CD33 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

    IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

    IHC staining of human tonsillitis using 84341-3-RR (same clone as 84341-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84341-3-PBS in a different storage buffer formulation.

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CD33 Recombinant monoclonal antibody, PBS Only

CD33 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84341-3-PBS

产品说明书

CloneNo. 241711D2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

241711D2, CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84341-3-PBS targets CD33 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2138

Product name: Recombinant Human CD33 protein (rFc Tag) (HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 18-259 aa of BC028152

Sequence: DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISGDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD33 molecule
别名 241711D2, CD33 molecule, gp67, p67, Sialic acid-binding Ig-like lectin 3
计算分子量 364 aa, 40 kDa
观测分子量55-75 kDa
GenBank蛋白编号BC028152
基因名称 CD33
Gene ID (NCBI) 945
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP20138
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.