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验证数据展示

  • WB analysis using 10343-1-AP (same clone as 10343-1-PBS)

    WB analysis using 10343-1-AP (same clone as 10343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10343-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

    IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

    IHC staining of mouse kidney using 10343-1-AP (same clone as 10343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10343-1-AP (CD320 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10343-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CD320 Polyclonal antibody, PBS Only

CD320 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10343-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

8D6, 8D6A, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10343-1-PBS targets CD320 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0344

Product name: Recombinant human CD320 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 84-280 aa of BC000668

Sequence: SDEEECRIEPCTQKGQCPPPPGLPCPCTGVSDCSGGTDKKLRNCSRLACLAGELRCTLSDDCIPLTWRCDGHPDCPDSSDELGCGTNEILPEGDATTMGPPVTLESVTSLRNATTMGPPVTLESVPSVGNATSSSAGDQSGSPTAYGVIAAAAVLSASLVTATLLLLSWLRAQERLRPLGLLVAMKESLLLSEQKTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD320 molecule
别名 8D6, 8D6A, FDC-signaling molecule 8D6, FDC-SM-8D6, TCblR
计算分子量 29 kDa
观测分子量 60-70 kDa, 35-40 kDa
GenBank蛋白编号BC000668
基因名称 CD320
Gene ID (NCBI) 51293
RRIDAB_2074223
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NPF0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa. This antibody raised against 84-280aa of human CD320 protein detects bands at 35-40 kDa and 60-70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

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