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验证数据展示

  • IF Staining of HUVEC using 65268-1-Ig (same clone as 65268-1-PBS)

    IF Staining of HUVEC using 65268-1-Ig (same clone as 65268-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (65268-1-Ig, Clone: WM-59 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). This data was developed using the same antibody clone with 65268-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65268-1-Ig (same clone as 65268-1-PBS)

    FC experiment of human PBMCs using 65268-1-Ig (same clone as 65268-1-PBS)

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD31 (65268-1-Ig, Clone:WM-59) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated. This data was developed using the same antibody clone with 65268-1-PBS in a different storage buffer formulation.

Anti-Human CD31 (WM-59), PBS Only

CD31 Monoclonal Antibody for IF/ICC, FC
Cat No. 65268-1-PBS

产品说明书

CloneNo. WM-59

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

IF/ICC, FC

别名

EndoCAM, GPIIA, GPIIA', PECA1, PECAM 1

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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65268-1-Ig

Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

产品信息

65268-1-PBS targets CD31 in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原

N/A

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule
别名 EndoCAM, GPIIA, GPIIA', PECA1, PECAM 1
计算分子量 83 kDa
GenBank蛋白编号BC022512
基因名称 CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP16284
储存缓冲液 PBS Only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

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