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验证数据展示

  • WB analysis using 32387-1-AP

    WB analysis using 32387-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32387-1-AP (CD31 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 32387-1-AP

    IHC staining of mouse liver using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 32387-1-AP

    IHC staining of mouse liver using 32387-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32387-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD31 Polyclonal antibody

CD31 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32387-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

PECAM-1, Pecam1, PECAM 1, Pecam

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inbEnd.3 cells
Positive IHC detected inmouse liver tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32387-1-AP targets CD31 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 platelet/endothelial cell adhesion molecule 1
别名 PECAM-1, Pecam1, PECAM 1, Pecam
计算分子量81 kDa
观测分子量120 kDa
GenBank蛋白编号NM_001305157.1
基因名称 CD31
Gene ID (NCBI) 18613
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ08481-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
WB protocol for CD31 antibody 32387-1-APDownload protocol
IHC protocol for CD31 antibody 32387-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols