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验证数据展示

  • WB analysis of MCF-7 using 30798-1-AP

    WB analysis of MCF-7 using 30798-1-AP

    WB result of CD300LD antibody (30798-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD300LD transfected MCF-7 cells.

  • WB analysis using 30798-1-AP

    WB analysis using 30798-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30798-1-AP (CD300LD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CD300LD Polyclonal antibody

CD300LD Polyclonal Antibody for WB, ELISA
Cat No. 30798-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

CMRF35A4, CMRF35 like molecule 4, CMRF35 A4, CLM 4, CD300D

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30798-1-AP targets CD300LD in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CD300LD fusion protein Ag29307 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD300 molecule-like family member d
别名 CMRF35A4, CMRF35 like molecule 4, CMRF35 A4, CLM 4, CD300D
计算分子量 22 kDa
观测分子量40-43 kDa
GenBank蛋白编号NM_001115152
基因名称 CD300LD
Gene ID (NCBI) 100131439
RRIDAB_3669758
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXZ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD300LD, also known as CD300D and CMRF35A4, is a member of the CD300 family. The CD300 family of molecules modulates a broad and diverse range of immune cell processes via their paired activating and inhibitory receptor functions. CD300LD is a 194 amino acid single-pass type I membrane protein containing an Ig-like V-type (immunoglobulin-like) domain. The apparent molecular weight is greater than the calculated molecular weight of 22 kDa, probably due to post-translational modification, presumably by glycosylation.

实验方案

Product Specific Protocols
WB protocol for CD300LD antibody 30798-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols