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验证数据展示

  • IHC staining of human tonsillitis using 32141-1-AP

    IHC staining of human tonsillitis using 32141-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32141-1-AP (CD300C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 32141-1-AP

    IHC staining of human tonsillitis using 32141-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 32141-1-AP (CD300C antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD300c Polyclonal antibody

CD300c Polyclonal Antibody for IHC, ELISA
Cat No. 32141-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

CMRF35, CMRF 35A, CMRF 35, CLM 6, CD300c molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32141-1-AP targets CD300c in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD300c molecule
别名 CMRF35, CMRF 35A, CMRF 35, CLM 6, CD300c molecule
计算分子量 224 aa, 25 kDa
GenBank蛋白编号BC022279
基因名称 CD300C
Gene ID (NCBI) 10871
RRIDAB_3670205
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ08708
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD300c is a member of the CD300 family of receptors, which are type I transmembrane proteins expressed in both myeloid and lymphoid lineages and modulate immune responses by their stimulatory and inhibitory capabilities (PMID: 30485414). CD300c, also known as CLM-6, CMRF-35 and IgSF16, is composed of an IgV-like extracellular domain, a transmembrane domain and a short cytoplasmic tail. It is expressed on the surface of monocytes, macrophages, dendritic cells, neutrophils, some T and B lymphocytes (PMID: 1349532; 30498497). CD300c has been reported as an activating receptor expressed on monocytes and that it has a potential role in inflammatory responses (PMID: 23571507). It has also been identified as a T cell co-inhibitory molecule (PMID: 30498497).

实验方案

Product Specific Protocols
IHC protocol for CD300c antibody 32141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols