验证数据展示
经过测试的应用
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
32141-1-AP targets CD300c in IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | Recombinant protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | CD300c molecule |
别名 | CMRF35, CMRF 35A, CMRF 35, CLM 6, CD300c molecule |
计算分子量 | 224 aa, 25 kDa |
GenBank蛋白编号 | BC022279 |
基因名称 | CD300C |
Gene ID (NCBI) | 10871 |
RRID | AB_3670205 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity Purification |
UNIPROT ID | Q08708 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD300c is a member of the CD300 family of receptors, which are type I transmembrane proteins expressed in both myeloid and lymphoid lineages and modulate immune responses by their stimulatory and inhibitory capabilities (PMID: 30485414). CD300c, also known as CLM-6, CMRF-35 and IgSF16, is composed of an IgV-like extracellular domain, a transmembrane domain and a short cytoplasmic tail. It is expressed on the surface of monocytes, macrophages, dendritic cells, neutrophils, some T and B lymphocytes (PMID: 1349532; 30498497). CD300c has been reported as an activating receptor expressed on monocytes and that it has a potential role in inflammatory responses (PMID: 23571507). It has also been identified as a T cell co-inhibitory molecule (PMID: 30498497).
实验方案
Product Specific Protocols | |
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IHC protocol for CD300c antibody 32141-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |