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验证数据展示

  • WB analysis using 87861-1-RR (same clone as 87861-1-PBS)

    WB analysis using 87861-1-RR (same clone as 87861-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87861-1-RR (CD3 gamma antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87861-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 87861-1-RR (same clone as 87861-1-PBS)

    IHC staining of mouse spleen using 87861-1-RR (same clone as 87861-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 87861-1-RR (CD3 gamma antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87861-1-PBS in a different storage buffer formulation.

  • Affinity of 87861-1-RR

    Affinity of 87861-1-RR

    Biolayer interferometry (BLI) kinetic assays of 87861-1-RR against Mouse CD3 gamma were performed. The affinity constant is 0.825 nM.

Mouse CD3 gamma Recombinant monoclonal antibody, PBS Only

CD3 gamma Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 87861-1-PBS

产品说明书

CloneNo. 253421H4

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, Indirect ELISA

别名

CD3g, T-cell receptor T3 gamma chain, T-cell surface glycoprotein CD3 gamma chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87861-1-PBS targets CD3 gamma in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg1402

Product name: Recombinant mouse CD3 gamma protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 23-116 aa of NM_009850.2

Sequence: QTNKAKNLVQVDGSRGDGSVLLTCGLTDKTIKWLKDGSIISPLNATKNTWNLGNNAKDPRGTYQCQGAKETSNPLQVYYRMCENCIELNIGTIS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD3 antigen, gamma polypeptide
别名 CD3g, T-cell receptor T3 gamma chain, T-cell surface glycoprotein CD3 gamma chain
计算分子量20 kDa
观测分子量20 kDa
GenBank蛋白编号NM_009850.2
基因名称 Cd3g
Gene ID (NCBI) 12502
RRIDAB_3745795
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP11942
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

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