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验证数据展示

  • WB analysis using 31889-1-AP

    WB analysis using 31889-1-AP

    Rat thymus tissue was subjected to SDS PAGE followed by western blot with 31889-1-AP (CD3 epsilon/CD3e antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 31889-1-AP

    IHC staining of rat spleen using 31889-1-AP

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31889-1-AP (CD3 epsilon antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD3 epsilon Polyclonal antibody

CD3 epsilon Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31889-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

rat

应用

WB, IHC, ELISA

别名

CD3e, T-cell surface glycoprotein CD3 epsilon chain

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat thymus tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31889-1-AP targets CD3 epsilon in WB, IHC, ELISA applications and shows reactivity with rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 rat
免疫原

CatNo: Eg1450

Product name: Recombinant Rat CD3 epsilon protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 24-103 aa of NP_001101610.2

Sequence: IEDTVTDDGAQKQYEVSISGTSVELTCPLENEDNLKWEKNDKVLPDKNEKHLVLEDFSEVKDSGYYVCYTESSRKNTYLY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD3 molecule, epsilon polypeptide
别名 CD3e, T-cell surface glycoprotein CD3 epsilon chain
计算分子量22kDa
观测分子量25 kDa
GenBank蛋白编号NP_001101610.2
基因名称 Cd3e
Gene ID (NCBI) 315609
RRIDAB_3670133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDA6J423
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This polyclonal antibody was raised against the epsilon chain of rat CD3 molecule.

实验方案

Product Specific Protocols
IHC protocol for CD3 epsilon antibody 31889-1-APDownload protocol
WB protocol for CD3 epsilon antibody 31889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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