验证数据展示
经过测试的应用
| Positive WB detected in | rat thymus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31889-1-AP targets CD3 epsilon in WB, ELISA applications and shows reactivity with rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | rat |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD3 molecule, epsilon polypeptide |
| 别名 | |
| 计算分子量 | 22kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | NP_001101610.2 |
| 基因名称 | Cd3e |
| Gene ID (NCBI) | 315609 |
| RRID | AB_3670133 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657). This polyclonal antibody was raised against the epsilon chain of rat CD3 molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD3 epsilon antibody 31889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |