CoraLite® Plus 647 Anti-Mouse CD3 (17A2)
CD3 Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
17A2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | mouse splenocytes |
Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65077-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-65077 targets CD3 in IF/ICC, FC applications and shows reactivity with Mouse samples.
Tested Applications | IF/ICC, FC Application Description |
Tested Reactivity | Mouse |
Immunogen | γδTCR-positive T-T hybridoma D1 种属同源性预测 |
Host / Isotype | Rat / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | CD3 antigen, epsilon polypeptide |
Synonyms | AI504783, CD3, Cd3e, CD3epsilon, T3e |
GenBank Accession Number | BC098236 |
Gene Symbol | CD3ε |
Gene ID (NCBI) | 12501 |
RRID | AB_2883666 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P22646 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 647 CD3 antibody CL647-65077 | Download protocol |
FC protocol for CL Plus 647 CD3 antibody CL647-65077 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |