• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Mouse CD3ε (500-A2)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

500-A2

Cat No : 65061-1-Ig

Print datasheet

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

验证数据展示

  • FC experiment of mouse splenocytes using 65061-1-Ig

    FC experiment of mouse splenocytes using 65061-1-Ig

    1X10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD3ε (65061-1-Ig, Clone: 500-A2) and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (green), or stained with 0.5 ug Armenian Hamster IgG isotype Control and FITC Goat anti-hamster (Armenian) IgG Antibody at dilution 1:200 (black). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65061-1-Ig targets CD3ε in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen Immune complexes isolated from C6VL-BS thymoma cells 种属同源性预测
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Full Name CD3 antigen, epsilon polypeptide
Synonyms AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e
GenBank Accession NumberBC098236
Gene Symbol Cd3e
Gene ID (NCBI) 12501
RRIDAB_2918369
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP22646
Storage Buffer Borate buffered saline, pH 8.2.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

实验方案

Product Specific Protocols
FC protocol for CD3ε antibody 65061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols