验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32930-1-AP targets CD2BP2 in applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38662 Product name: Recombinant human CD2BP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-341 aa of NM_001243646.1 Sequence: MFAEELAEEELETPTPTQRGEAESRGDGLVDVMWEYKWENTGDAELYGPFTSAQMQTWVSEGYFPDGVYCRKLDPPGGQFYNSKRIDFDLYT* 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD2 (cytoplasmic tail) binding protein 2 |
| 别名 | CD2 antigen cytoplasmic tail-binding protein 2, CD2 cytoplasmic domain-binding protein 2, CD2 Cytoplasmic Tail Binding Protein 2, CD2 tail-binding protein 2, KIAA1178 |
| 计算分子量 | 38kDa，341aa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | NM_001243646.1 |
| 基因名称 | CD2BP2 |
| Gene ID (NCBI) | 10421 |
| RRID | AB_3742794 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O95400 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD2BP2 (CD2-binding protein 2) is a multifunctional protein that was initially identified as a binding partner of the T-cell surface antigen CD2. It binds to the cytoplasmic tail of CD2 through its C-terminal GYF domain in the cytoplasm, regulating CD2-mediated T-lymphocyte activation. Additionally, CD2BP2 is a component of the U5 small nuclear ribonucleoprotein complex (snRNP), participating in the RNA splicing process. Studies have shown that CD2BP2 plays a crucial role in embryonic development; its absence leads to delayed embryonic growth, vascularization defects, and death by embryonic day 10.5. Specific depletion of CD2BP2 in renal podocytes results in proteinuria and ultimately fatal renal failure.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD2BP2 antibody 32930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
