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APC Anti-Human PD-1/CD279 (EH12.2H7)

PD-1/CD279 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Non-human primates, Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

EH12.2H7

Cat No : APC-65168

Print datasheet

Synonyms

CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

验证数据展示

  • FC experiment of human peripheral blood mononuclear cells using APC-65168

    FC experiment of human peripheral blood mononuclear cells using APC-65168

    1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and 10.00 ul/test APC Anti-Human PD-1/CD279 (APC-65168, Clone:EH12.2H7). Cells were not fixed.

  • FC experiment of human peripheral blood mononuclear cells using APC-65168

    FC experiment of human peripheral blood mononuclear cells using APC-65168

    1X10^6 human peripheral blood mononuclear cells (PBMCs) were surface stained with FITC Anti-Human CD3 (FITC-65151, clone UCHT1) and APC mouse IgG1 isotype control antibody. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood mononuclear cells cells

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65168 targets PD-1/CD279 in FC applications and shows reactivity with Non-human primates, Human samples.

Tested Applications FC
Tested Reactivity Non-human primates, Human
Immunogen Recombinant human CD279 protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, hPD 1, hPD l, PD1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Calculated Molecular Weight 288 aa, 32 kDa
GenBank Accession NumberBC074740
Gene Symbol PDCD1
Gene ID (NCBI) 5133
RRIDAB_2883038
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDQ15116
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).

实验方案

Product Specific Protocols
FC protocol for APC PD-1/CD279 antibody APC-65168Download protocol
Standard Protocols
Click here to view our Standard Protocols