验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, PC-3 cells |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue, human prostate cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66481-1-Ig targets B7-H3/CD276 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6006 Product name: Recombinant human CD276 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 41-181 aa of BC062581 Sequence: LVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD276 molecule |
| 别名 | CD276, B7-H3, B7H3, B7 homolog 3, B7 H3 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC062581 |
| 基因名称 | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2881847 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5ZPR3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| IHC protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| WB protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Enhancement of CAR-T cell activity against cholangiocarcinoma by simultaneous knockdown of six inhibitory membrane proteins | ||
Cancer Lett CD276 promotes CD36-mediated lipid metabolism and confers resistance to tyrosine kinase inhibitors.
| ||
J Immunother Cancer Multiomic analysis of colorectal adenocarcinoma reveals a new subtype of myofibroblastic cancer-associated fibroblasts that express high levels of B7-H3 and have poor-prognosis value. | ||
J Immunother Cancer Engineered oncolytic virus expressing B7H3-targeting BiTE enhances antitumor T-cell immune response |
