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验证数据展示

  • FC experiment of human peripheral blood lymphocytes using CL647-65132

    FC experiment of human peripheral blood lymphocytes using CL647-65132

    1X10^6 human peripheral blood lymphocytes were surface stained with 5.00 ul CoraLite® Plus 647-conjugated Anti-Human CD27 (CL647-65132, Clone: O323) (red) or isotype control antibody (blue). Cells were not fixed.

CoraLite® Plus 647 Anti-Human CD27 (O323)

CD27 Monoclonal Antibody for FC
Cat No. CL647-65132

产品说明书

CloneNo. O323

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

CD27 antigen, CD27 molecule, CD27L receptor, O323, S152

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
For other applications, we recommend the unconjugated version of this antibody, 65132-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65132 targets CD27 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 CD27 molecule
别名 CD27 antigen, CD27 molecule, CD27L receptor, O323, S152
计算分子量 29 kDa
GenBank蛋白编号BC012160
基因名称 CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
RRIDAB_2883699
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP26842
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD27 antibody CL647-65132Download protocol
Standard Protocols
Click here to view our Standard Protocols