• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using APC-65096

    FC experiment of human PBMCs using APC-65096

    1x10^6 untreated (left) or PHA-treated (3 days) (right) human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) and CoraLite® Plus 488 Anti-Human CD4. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65096

    FC experiment of human PBMCs using APC-65096

    1x10^6 PHA-treated (3 days) human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) (red) or APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21)(blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) (black, dashed). Cells were not fixed. Lymphocytes were gated.

APC Anti-Human CD25/IL-2RA (BC96)

CD25/IL-2RA Monoclonal Antibody for FC
Cat No. APC-65096

产品说明书

CloneNo. BC96

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

缓冲液配方:  PBS, Azide, Gelatin
PBS, Azide, Gelatin
偶联物:  APC
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65096 targets CD25/IL-2RA in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原

N/A

种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 interleukin 2 receptor, alpha
别名 CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha
计算分子量 272 aa, 31 kDa
GenBank蛋白编号BC156627
基因名称 IL2RA
Gene ID (NCBI) 3559
RRIDAB_2882988
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式Liquid
纯化方式Affinity purification
UNIPROT IDP01589
储存缓冲液 PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

实验方案

Product Specific Protocols
FC protocol for APC CD25/IL-2RA antibody APC-65096Download protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...