TEM1 Monoclonal antibody
TEM1 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse and More (1)
Applications
IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
1F9B4
验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, COLO 320 cells, bEnd.3 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60170-1-Ig targets TEM1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TEM1 fusion protein Ag13334 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD248 molecule, endosialin |
| Synonyms | CD164L1, CD248, CD248 molecule, endosialin, Endosialin, TEM1, Tumor endothelial marker 1 |
| Calculated Molecular Weight | 757 aa, 81 kDa |
| Observed Molecular Weight | 150-160 kDa |
| GenBank Accession Number | BC051340 |
| Gene Symbol | CD248 |
| Gene ID (NCBI) | 57124 |
| RRID | AB_10694834 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9HCU0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TEM1 (Tumor endothelial marker 1), also named as CD248, Endosialin and CD164L1, is a C-type lectin-like domain (CTLD) containing type I transmembrane glycoprotein. It is now considered to be a highly selective marker for activated perivascular and stromal cells, detected in most cancers and at least some inflammatory disorders. CD248 plays a role in tumor angiogenesis. It is a potential diagnostic tool and therapeutic target of inflammatory and malignant disease. Two isoforms of human TEM1 exist. The calculated molecular weights of the two isoforms are 81 kDa and 46 kDa, respectively. Native TEM1 can be glycosylated, and the glycosylated form has a larger apparent molecular weight than 81 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TEM1 antibody 60170-1-Ig | Download protocol |
| IHC protocol for TEM1 antibody 60170-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer | ||
Proc Natl Acad Sci U S A A membrane protein display platform for receptor interactome discovery. | ||
Oncotarget Generation of a novel Antibody-Drug Conjugate targeting endosialin: potent and durable antitumor response in sarcoma. | ||
Sci Rep Tumor endothelial marker 1 is upregulated in heart after cardiac injury and participates in cardiac remodeling. | ||
Front Cardiovasc Med Soluble tumor endothelial marker 1 in heart failure with reduced ejection fraction: A pilot study | ||
EBioMedicine CD248 promotes insulin resistance by binding to the insulin receptor and dampening its insulin-induced autophosphorylation
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