CD247 Polyclonal antibody

CD247 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12837-2-AP

Print datasheet

Synonyms

CD247, CD247 molecule, CD3 ZETA, CD3H, CD3Q, CD3Z, T cell receptor T3 zeta chain, T3Z, TCRZ



经过测试的应用

Positive WB detected inJurkat cells, human spleen tissue, mouse spleen tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CD247 Ready-To-Use IHC Kit. CD247 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12837-2-AP targets CD247 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB,ELISA Application Description
Cited ApplicationsWB, IP, FC, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CD247 fusion protein Ag3889 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD247 molecule
Synonyms CD247, CD247 molecule, CD3 ZETA, CD3H, CD3Q, CD3Z, T cell receptor T3 zeta chain, T3Z, TCRZ
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC025703
Gene Symbol CD247
Gene ID (NCBI) 919
RRIDAB_2244279
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20963
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD247, also known as CD3Z (T-cell surface glycoprotein CD3 zeta chain), belongs to the CD3Z/FCER1G family. CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

实验方案

Product Specific Protocols
WB protocol for CD247 antibody 12837-2-APDownload protocol
IHC protocol for CD247 antibody 12837-2-APDownload protocol
IF protocol for CD247 antibody 12837-2-APDownload protocol
IP protocol for CD247 antibody 12837-2-APDownload protocol
FC protocol for CD247 antibody 12837-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

The Transferrin Receptor-Directed CAR for the Therapy of Hematologic Malignancies.

Authors - Zilong Guo
mouseWB

PLoS One

Liver Sinusoidal Endothelial Cell-Mediated CD8 T Cell Priming Depends on Co-Inhibitory Signal Integration over Time.

Authors - Julita Kaczmarek
humanIHC

Front Immunol

Distinct immune and inflammatory response patterns contribute to the identification of poor prognosis and advanced clinical characters in bladder cancer patients

Authors - Zhenglin Chang
humanFC

Cytotherapy

Bruton tyrosine kinase inhibitors preserve anti-CD19 chimeric antigen receptor T-cell functionality and reprogram tumor micro-environment in B-cell lymphoma

Authors - Wenjing Luo
humanWB,IP

Cell Rep

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance

Authors - Xueliang Gao
mouseWB

Nat Commun

Cell volume controlled by LRRC8A-formed volume-regulated anion channels fine-tunes T cell activation and function

Authors - Yuman Wang