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验证数据展示

  • WB analysis using 84086-1-RR

    WB analysis using 84086-1-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84086-1-RR

    Affinity and Kinetic Characterization of 84086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

CD244 Recombinant monoclonal antibody

CD244 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 84086-1-RR

产品说明书

CloneNo. 241260C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

SLAMF4, 241260C6, 2B4, h2B4, NAIL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84086-1-RR targets CD244 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg32087

Product name: Recombinant Human CD244 protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 22-221 aa of BC053985

Sequence: CQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWENGSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATFQVFVFDKVEKPRLQGQGKILDRGRCQVALSCLVSRDGNVSYAWYRGSKLIQTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLTQDCQNAHQEFR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD244 molecule, natural killer cell receptor 2B4
别名 SLAMF4, 241260C6, 2B4, h2B4, NAIL
计算分子量 365 aa, 41 kDa
观测分子量70 kDa
GenBank蛋白编号BC053985
基因名称 CD244
Gene ID (NCBI) 51744
RRIDAB_3671654
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

实验方案

Product Specific Protocols
IHC protocol for CD244 antibody 84086-1-RRDownload protocol
WB protocol for CD244 antibody 84086-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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