验证数据展示
经过测试的应用
| Positive WB detected in | NK-92 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
16677-1-AP targets CD244 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag9860 Product name: Recombinant human CD244 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-220 aa of BC053985 Sequence: GCQGSADHVVSISGVPLQLQPNSIQTKVDSIAWKKLLPSQNGFHHILKWENGSLPSNTSNDRFSFIVKNLSLLIKAAQQQDSGLYCLEVTSISGKVQTATFQVFVFDKVEKPRLQGQGKILDRGRCQVALSCLVSRDGNVSYAWYRGSKLIQTAGNLTYLDEEVDINGTHTYTCNVSNPVSWESHTLNLTQDCQNAHQEF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CD244 molecule, natural killer cell receptor 2B4 |
| 别名 | 2B4, CD244, h2B4, NAIL, NKR2B4, SLAMF4 |
| 计算分子量 | 365 aa, 41 kDa |
| 观测分子量 | 60-70 kDa |
| GenBank蛋白编号 | BC053985 |
| 基因名称 | CD244 |
| Gene ID (NCBI) | 51744 |
| RRID | AB_2878297 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BZW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD244 antibody 16677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Med A case report of clonal EBV-like memory CD4+ T cell activation in fatal checkpoint inhibitor-induced encephalitis. | ||
Nat Commun Pluripotency and X chromosome dynamics revealed in pig pre-gastrulating embryos by single cell analysis. |