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验证数据展示

  • IHC staining of mouse placenta using 30864-1-AP

    IHC staining of mouse placenta using 30864-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30864-1-AP (CD235a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 30864-1-AP

    IHC staining of mouse placenta using 30864-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 30864-1-AP (CD235a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Glycophorin A/CD235a Polyclonal antibody

Glycophorin A/CD235a Polyclonal Antibody for IHC, ELISA
Cat No. 30864-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IHC, ELISA

别名

glycophorin A, Gypa, GPA, Glycophorin-A, CD235a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30864-1-AP targets Glycophorin A/CD235a in IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Ag33770

Product name: Recombinant mouse CD235a protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-108 aa of NM_010369

Sequence: MTESTAAVTTSGHSLTTTFHIPSSQHYQEEHSPSLSGSDSLLQITTPVVASTVGNPNQHSATMSTPAIHVSTYHTAPTEVSAAFEEQPVSPHIGGMPSPIQHDFPALV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycophorin A
别名 glycophorin A, Gypa, GPA, Glycophorin-A, CD235a
计算分子量18kd
GenBank蛋白编号NM_010369
基因名称 Gypa
Gene ID (NCBI) 14934
RRIDAB_3669769
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP14220
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)

实验方案

Product Specific Protocols
IHC protocol for Glycophorin A/CD235a antibody 30864-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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