验证数据展示
经过测试的应用
| Positive WB detected in | Transfected with Mouse CD232/PLXNC1 HEK-293T cells, mouse brain tissue, HEK-293T cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87759-2-RR targets CD232/PLXNC1 in WB, IHC, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg6304 Product name: Recombinant mouse CD232/PLXNC1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 35-950 aa of NM_018797.3 Sequence: ADEPVWRSEQAIGAIAASRADGVFVASGSCLDQLDYSLKNRLSRLYRDQAGNCTEPVSLAPPARPRPGSSFSKLLLPYREGATGLEGLLLTGWTFDRGACEVRPLGNLNRSSLRNGTEVVSCHPQGSTAGVVYRASGTDLWYLAVAATYVLPEPETANRCNPAASDRDTAIALKNTEGRSLATQELGRLKLRGSAGSLHFVDAFLWNGSVYFPYYPYNYTSGAATGWPSMARIAQSTEVLFQGQAALDCDHGHPEGRRLLLSSSLVEAVDIWAGVFSAATGEGQERRSPATTALCLFRMSEIQAHARSCSWDFQATEHNCKEGDRPERVQPIASSTLIHSDLTSVYGTVVMNRTVLFLGTGDGQLLKVVLGENLTSNCPEVIYEIKEETPVFYKLVPHPMKNIYIYLTAGKEVRRIPVANCSKRKSCSECLAAADPHCGWCLPLQRCTFQGDCTHAGSFENWLDISSGPKKCPKIQILRSLRERTTVTIVGSISARHSECVVKNADTGKLLCQGRSQLNWTCACNIPSRPSYNVLVVNATFSFPSWNLSERFNFTNCASLKECPACIRSGCAWCKRDKKCIHPFTPCEPSDYERNQELCQVAVEKSPKDSGGGRVKESKRNRTDGAVQVFYIKAIEPQKISTLGKSNVIVTGANFTQASNITMILRGTSTCERDVIRVSHVLNDTHMKFSLPSSRKEMKDVCIQFDGGTCSSAGALSYIALPHCSLIVPATTWISGGQNITIMGRNFDVIDNLIISHELKGNANVNINVSEYCAATFCRFLAPNLKSSKVRTNVAVKLRVQDTYLDCGTLQYLEDPRFTGYRVESEIDTELEVKIQKENDNFNISKDDIDITLFHGENKQFNCSFENITRNQDLTTILCKIKSIKNANTIASSSKKVRVKLGNLELYVEQESVPST 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | plexin C1 |
| 别名 | CD232, Plexin-C1, Plxnc1, Vespr |
| 计算分子量 | 176 kDa |
| 观测分子量 | 220 kDa |
| GenBank蛋白编号 | NM_018797.3 |
| 基因名称 | Plxnc1 |
| Gene ID (NCBI) | 54712 |
| RRID | AB_3745737 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9QZC2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PlxnC1 in mouse encodes a semaphorin receptor that integrates extracellular guidance and immune cues through R-Ras/Rap1-GTPase regulation. Expressed in neurons, epithelial cells, and immune cells, it governs axon targeting, olfactory circuit formation, and inflammatory responses. Loss of PlxnC1 disrupts olfactory axon sorting and modifies immune signaling, underscoring its conserved role in neurodevelopmental patterning and immune homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD232/PLXNC1 antibody 87759-2-RR | Download protocol |
| WB protocol for CD232/PLXNC1 antibody 87759-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
