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验证数据展示

  • WB analysis using 17842-1-AP

    WB analysis using 17842-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 17842-1-AP (CD226 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 17842-1-AP

    IHC staining of human tonsillitis using 17842-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17842-1-AP (CD226 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD226 Polyclonal antibody

CD226 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17842-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

DNAX accessory molecule 1, DNAM-1, DNAM1, DNAM 1, CD226 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuman peripheral blood platelets, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17842-1-AP targets CD226 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 CD226 fusion protein Ag12206 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD226 molecule
别名 DNAX accessory molecule 1, DNAM-1, DNAM1, DNAM 1, CD226 molecule
计算分子量 336 aa, 39 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC074787
基因名称 CD226
Gene ID (NCBI) 10666
ENSEMBL Gene IDENSG00000150637
RRIDAB_2072623
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD226 (DNAM-1) is a ~65 kDa glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. CD226 mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Interactions of CD226 and its ligands, CD155 and CD112, induce NK and T cell-mediated cytotoxicity and cytokine secretion (PMID: 15039383).

实验方案

Product Specific Protocols
WB protocol for CD226 antibody 17842-1-APDownload protocol
IHC protocol for CD226 antibody 17842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Adv Sci (Weinh)

Deciphering the Molecular Characteristics of Human Idiopathic Nonobstructive Azoospermia from the Perspective of Germ Cells

Authors - Yidong Chen
View Article