• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84265-2-RR (same clone as 84265-2-PBS)

    WB analysis using 84265-2-RR (same clone as 84265-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84265-2-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84265-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01173-3

    Sandwich ELISA standard curve of MP01173-3

    Sandwich ELISA standard curve of MP01173-3, Human CD22 Recombinant Matched Antibody Pair - PBS only. 84265-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02324. 84265-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

  • Sample test of MP01173-3

    Sample test of MP01173-3

    The mean CD22 concentration was determined to be 445.39 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load and 185.59 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load.

  • Sample test of MP01173-3

    Sample test of MP01173-3

    Serum of six individual healthy human donors was measured. The CD22 concentration of detected samples was determined to be 0.36 ng/mL with a range of 0.25-0.54 ng/mL.

  • Cytometric bead array standard curve of MP01173-3

    Cytometric bead array standard curve of MP01173-3

    Cytometric bead array standard curve of MP01173-3, CD22 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84265-4-PBS. Detection antibody: 84265-2-PBS. Standard: RP02324. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84265-2-RR

    Affinity and Kinetic Characterization of 84265-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84265-2-RR against Human CD22 were performed. The affinity constant is 61.3 pM.

CD22 Recombinant monoclonal antibody, PBS Only (Detector)

CD22 Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84265-2-PBS

产品说明书

CloneNo. 241314G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

241314G10, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy CD22 Ready-To-Use IHC Kit. CD22 primary antibody included.

产品信息

84265-2-PBS targets CD22 as part of a matched antibody pair:

MP01173-3: 84265-4-PBS capture and 84265-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原

Recombinant Protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD22 molecule
别名 241314G10, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule
计算分子量 847 aa, 95 kDa
观测分子量130-140 kDa
GenBank蛋白编号BC109306
基因名称 CD22
Gene ID (NCBI) 933
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP20273
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

||
New chat Able
正在加载，请稍候...