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验证数据展示

  • IHC staining of human tonsillitis using 28206-1-AP

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28206-1-AP

    IHC staining of human tonsillitis using 28206-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28206-1-AP (CD21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 28206-1-AP

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human tonsillitis using 28206-1-AP

    IF Staining of human tonsillitis using 28206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD21 antibody (28206-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CD21 Polyclonal antibody

CD21 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 28206-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

IHC, IF-P, ELISA

别名

C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28206-1-AP targets CD21 in IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag27971

Product name: Recombinant human CR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 660-718 aa of BC136394

Sequence: GCQSPPGLHHGRHTGGNTVFFVSGMTVDYTCDPGYLLVGNKSIHCMPSGNWSPSAPRCE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complement component (3d/Epstein Barr virus) receptor 2
别名 C3DR, CD21, Complement C3d receptor, Complement receptor type 2, CR2, EBV receptor, Epstein Barr virus receptor, SLEB9
计算分子量 1092 aa, 119 kDa
GenBank蛋白编号BC136394
基因名称 CD21
Gene ID (NCBI) 1380
ENSEMBL Gene IDENSG00000117322
RRIDAB_2881087
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20023
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD21, also known as complement receptor type 2 (CR2), complement C3d receptor, and Epstein-Barr virus receptor, is a transmembrane protein that contains a small cytoplasmic domain, a transmembrane region and an extracellular domain consisting of 15 tandem short consensus repeat sequences (PMID: 6230668; 2551147). It is expressed on B cells, follicular dendritic cells, thymocytes and a subset of peripheral T cells (PMID: 26119182). CD21 binds complement fragments C3d, C3dg and iC3b and acts as a receptor for the Epstein-Barr virus (PMID: 7753047). On B cells, together with CD19 and CD81, CD21 forms a complex that functions as a co-receptor to the BCR (PMID: 7542009). It is involved in B cells activation and functions.

实验方案

Product Specific Protocols
IF protocol for CD21 antibody 28206-1-APDownload protocol
IHC protocol for CD21 antibody 28206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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