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验证数据展示

  • WB analysis of human placenta using 25404-1-AP

    WB analysis of human placenta using 25404-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25404-1-AP (CD209 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 25404-1-AP

    IF Staining of human tonsillitis using 25404-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD209 antibody (25404-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CD209 Polyclonal antibody

CD209 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 25404-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF-P, ELISA

别名

CDSIGN, CLEC4L, C-type lectin domain family 4 member L, DC SIGN, DC SIGN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy® CD209 Ready-To-Use IHC Kit. CD209 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25404-1-AP targets CD209 in WB, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag21863

Product name: Recombinant human CD209 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-380 aa of BC110615

Sequence: MASACPGSDFTSIHSEEEQLRGLGFRQTRGYKSLAVSKVPSSISQEQSRQDAIYQNLTQLKAAVGELSEKSKLQEIYQELTQLKAAVGELPEKSKLQEIYQELTRLKAAVGELPEKSKLQEIYQELTWLKAAVGELPEKSKMQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTRLKAAVGELPEKSKQQEIYQELTQLKAAVERLCHPCPWEWTFFQGNCYFMSNSQRNWHDSITACKEVGAQLVVIKSAEEQNFLQLQSSRSNRFTWMGLSDLNQEGTWQWVDGSPLLPSFKQYWNRGEPNNVGEEDCAEFSGNGWNDDKCNLAKFWICKKSAASCSRDEEQFLSPAPATPNPPPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD209 molecule
别名 CDSIGN, CLEC4L, C-type lectin domain family 4 member L, DC SIGN, DC SIGN1
计算分子量 404 aa, 45.7 kDa
观测分子量 45 kDa
GenBank蛋白编号BC110615
基因名称 CD209
Gene ID (NCBI) 30835
RRIDAB_2880062
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NNX6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD209 (DC-SIGN, CLEC4L) is a C-type lectin receptor that is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, including bacteria, viruses, and parasites. CD209 is preferentially expressed on dendritic cells (DCs). It mediates transient adhesion of DCs with T cells. It has been reported that CD209 plays a role in capture and transmission of HIV from DC to T cells (PMID: 10721995).

实验方案

Product Specific Protocols
WB protocol for CD209 antibody 25404-1-APDownload protocol
IF protocol for CD209 antibody 25404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

SARS-CoV-2 exacerbates proinflammatory responses in myeloid cells through C-type lectin receptors and Tweety family member 2.

Authors - Qiao Lu
  • KD Validated
View Article
humanWB

Int J Mol Sci

Profiling of Early Immune Responses to Vaccination Using THP-1-Derived Dendritic Cells

Authors - Lei Ye
View Article
humanWB

J Neurooncol

Exploring the prognostic value of BRMS1 + microglia based on single-cell anoikis regulator patterns in the immunologic microenvironment of GBM

Authors - Songyun Zhao
View Article