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验证数据展示

  • WB analysis using 31444-1-AP

    WB analysis using 31444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31444-1-AP (CD200 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 31444-1-AP

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31444-1-AP

    IHC staining of mouse spleen using 31444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31444-1-AP (CD200 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD200 Polyclonal antibody

CD200 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31444-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IHC, ELISA

别名

OX2, Mox2, CD200 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse lung tissue, mouse spleen tissue, mouse thymus tissue, rat brain tissue
Positive IHC detected inmouse spleen tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31444-1-AP targets CD200 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD200 antigen
别名 OX2, Mox2, CD200 antigen
计算分子量31 kDa
观测分子量40-50 kDa
基因名称 Cd200
Gene ID (NCBI) 17470
RRIDAB_3669986
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO54901
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD200, also known as OX2, is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 3032785). It contains two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. CD200 is expressed on a variety of cell types, including thymocytes, B lymphocytes, a subset of T lymphocytes, follicular dendritic cells, neurons, and endothelial cells (PMID: 3032785; 10981966). CD200 binds to its receptor, CD200R, which is primarily expressed by myeloid and T cell lineage (PMID: 15187158). The CD200-CD200R interaction plays a role in immunosuppression and suppression of anti-tumor immune responses (PMID: 22020332).

实验方案

Product Specific Protocols
WB protocol for CD200 antibody 31444-1-APDownload protocol
IHC protocol for CD200 antibody 31444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols