CD2 Monoclonal antibody

CD2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3A10B2

Cat No : 60005-1-Ig

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Synonyms

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11



经过测试的应用

Positive WB detected inJurkat cells, human spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

60005-1-Ig targets CD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF
Tested Reactivity human
Cited Reactivityhuman, sheep
Immunogen CD2 fusion protein Ag0423 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD2 molecule
Synonyms CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11
Calculated Molecular Weight 351 aa, 39 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2881350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP06729
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
WB protocol for CD2 antibody 60005-1-IgDownload protocol
IHC protocol for CD2 antibody 60005-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,sheepIF

Exp Hematol

Fetal sheep support the development of hematopoietic cells in vivo from human induced pluripotent stem cells

Authors - Tomoyuki Abe