CD2 Polyclonal antibody
CD2 Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | Jurkat cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
产品信息
10299-1-AP targets CD2 in WB, IHC, FC,ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IHC, WB,ELISA |
Tested Reactivity | human |
Immunogen | CD2 fusion protein Ag0423 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CD2 molecule |
Synonyms | CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11 |
Calculated molecular weight | 351 aa, 39 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC033583 |
Gene symbol | CD2 |
Uniprot | P06729 |
Gene ID (NCBI) | 914 |
RRID | AB_2228874 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
实验方案
Product Specific Protocols | |
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WB protocol for CD2 antibody 10299-1-AP | Download protocol |
IHC protocol for CD2 antibody 10299-1-AP | Download protocol |
FC protocol for CD2 antibody 10299-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |