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  • Featured Product
  • KD/KO Validated

CD1d Monoclonal antibody

CD1d Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1C12F1

Cat No : 66257-1-Ig

Print datasheet

Synonyms

CD1A, CD1D, CD1d molecule, R3, R3G1

验证数据展示

  • WB analysis of Jurkat using 66257-1-Ig

    WB analysis of Jurkat using 66257-1-Ig

    WB result of CD1D antibody (66257-1-Ig, 1:500) with si-Control and si-CD1D transfected Jurkat cells.

  • WB analysis of human testis using 66257-1-Ig

    WB analysis of human testis using 66257-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66257-1-Ig (CD1D Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 66257-1-Ig

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human skin using 66257-1-Ig

    IHC staining of human skin using 66257-1-Ig

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66257-1-Ig (CD1d antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman testis tissue, Jurkat
Positive IHC detected inhuman skin tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66257-1-Ig targets CD1d in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen CD1d fusion protein Ag11108 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD1d molecule
Synonyms CD1A, CD1D, CD1d molecule, R3, R3G1
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa, 50-55 kDa
GenBank Accession NumberBC027926
Gene Symbol CD1D
Gene ID (NCBI) 912
RRIDAB_2881645
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15813
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD1d is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on NKT cells. When activated, NKT cells rapidly produce Th1 and Th2 cytokines. The molecular weight of unglycosylated CD1d is 38 kDa, while glycosylated form of CD1d is 48-55 kDa.

实验方案

Product Specific Protocols
WB protocol for CD1d antibody 66257-1-IgDownload protocol
IHC protocol for CD1d antibody 66257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols