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CD19 Polyclonal antibody

CD19 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27914-1-AP

Print datasheet

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

验证数据展示

  • WB analysis using 27914-1-AP

    WB analysis using 27914-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27914-1-AP (CD19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 27914-1-AP

    IP experiment of Raji using 27914-1-AP

    IP result of anti-CD19 (IP:27914-1-AP, 4ug; Detection:27914-1-AP 1:3000) with Raji cells lysate 1800 ug.

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IP detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27914-1-AP targets CD19 in WB, IP, ELISA applications and shows reactivity with Human samples.

Tested Applications IP, WB, ELISA
Tested Reactivity Human
Immunogen CD19 fusion protein Ag27404 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD19 molecule
Synonyms B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12
Calculated Molecular Weight 556 aa, 61 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
ENSEMBL Gene IDENSG00000177455
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD19 is a B cell-specific molecule that controls B cell activation by complexing with the BCR. CD19 is a member of the Ig superfamily and is the dominant component for the signaling complex on B cells that includes CD21, CD81, and CD22. Humans with CD19 deficiency have reduced proliferative responses to BCR stimulation in vitro and mount impaired Ab responses to vaccination.

实验方案

Product Specific Protocols
WB protocol for CD19 antibody 27914-1-APDownload protocol
IP protocol for CD19 antibody 27914-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols