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验证数据展示

  • WB analysis using 28304-1-AP

    WB analysis using 28304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28304-1-AP (CD180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 28304-1-AP

    IHC staining of human tonsillitis using 28304-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28304-1-AP (CD180 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28304-1-AP

    IHC staining of human tonsillitis using 28304-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28304-1-AP (CD180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD180 Polyclonal antibody

CD180 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28304-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CD180, CD180 antigen, CD180 molecule, LY64, Ly78, Lymphocyte antigen 64, RP105

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28304-1-AP targets CD180 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 CD180 fusion protein Ag28476 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD180 molecule
别名 CD180, CD180 antigen, CD180 molecule, LY64, Ly78, Lymphocyte antigen 64, RP105
计算分子量 661 aa, 74 kDa
观测分子量95-105 kDa
GenBank蛋白编号BC109069
基因名称 CD180
Gene ID (NCBI) 4064
RRIDAB_2881108
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99467
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CD180 antibody 28304-1-APDownload protocol
IHC protocol for CD180 antibody 28304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols