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CD180 Polyclonal antibody

CD180 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28304-1-AP

Print datasheet

Synonyms

CD180, CD180 antigen, CD180 molecule, LY64, Ly78, Lymphocyte antigen 64, RP105

验证数据展示

  • WB analysis using 28304-1-AP

    WB analysis using 28304-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28304-1-AP (CD180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 28304-1-AP

    IHC staining of human tonsillitis using 28304-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28304-1-AP (CD180 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 28304-1-AP

    IHC staining of human tonsillitis using 28304-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28304-1-AP (CD180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28304-1-AP targets CD180 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen CD180 fusion protein Ag28476 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD180 molecule
Synonyms CD180, CD180 antigen, CD180 molecule, LY64, Ly78, Lymphocyte antigen 64, RP105
Calculated Molecular Weight 661 aa, 74 kDa
Observed Molecular Weight95-105 kDa
GenBank Accession NumberBC109069
Gene Symbol CD180
Gene ID (NCBI) 4064
RRIDAB_2881108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99467
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CD180 antibody 28304-1-APDownload protocol
IHC protocol for CD180 antibody 28304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols