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验证数据展示

  • WB analysis using 87449-1-RR (same clone as 87449-1-PBS)

    WB analysis using 87449-1-RR (same clone as 87449-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87449-1-RR (ITB2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87449-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 87449-1-RR (same clone as 87449-1-PBS)

    IHC staining of human tonsillitis using 87449-1-RR (same clone as 87449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87449-1-RR (CD18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87449-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 87449-1-RR (same clone as 87449-1-PBS)

    IHC staining of human tonsillitis using 87449-1-RR (same clone as 87449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87449-1-RR (CD18 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87449-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 87449-1-RR (same clone as 87449-1-PBS)

    IF Staining of THP-1 using 87449-1-RR (same clone as 87449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells (postive) and HeLa cells (Negative) using CD18 antibody (87449-1-RR, Clone: 252742C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87449-1-PBS in a different storage buffer formulation.

  • Affinity of 87449-1-RR

    Affinity of 87449-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87449-1-RR against Human CD18 were performed. The affinity constant is below 1 pM.

CD18 Recombinant monoclonal antibody, PBS Only

CD18 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87449-1-PBS

产品说明书

CloneNo. 252742C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ITGB2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Integrin beta 2, Integrin beta-2, LAD

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87449-1-PBS targets CD18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg10481

Product name: Recombinant human ITB2 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-Twin-Strep-Tag

Domain: 10011434 of

Sequence: \

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
别名 ITGB2, Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta, Integrin beta 2, Integrin beta-2, LAD
观测分子量 90-100 kDa
基因名称 CD18
Gene ID (NCBI) 3689
ENSEMBL Gene IDENSG00000160255
RRIDAB_3745554
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP05107
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD18, also known as integrin beta-2, is a transmembrane protein that forms heterodimers with CD11a, CD11b, CD11c, CD11d (PMID: 1968349). CD11a/CD18 (LFA-1) is expressed by all leukocytes, while CD11b/CD18 (Mac-1) and CD11c/CD18 (p150,95) are normally restricted to expression on monocytes, macrophages, polymorphonuclear lymphocytes (PMNs), and natural killer cells (PMID: 1968349; 3109455; 10946284). CD18 plays an important role in mediating cell adhesion during the immune response, and defects of CD18 cause leukocyte adhesion deficiency (PMID: 3594570).

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