验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, NALM-6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87298-1-RR targets CD179b in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Eg3515 Product name: recombinant human IGLL1 protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 38-213 aa of BC012293 Sequence: LLRPTAASQSRALGPGAPGGSSRSSLRSRWGRFLLQRGSWTGPRCWPRGFQSKHNSVTHVFGSGTQLTVLSQPKATPSVTLFPPSSEELQANKATLVCLMNDFYPGILTVTWKADGTPITQGVEMTTPSKQSNNKYAASSYLSLTPEQWRSRRSYSCQVMHEGSTVEKTVAPAECS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | immunoglobulin lambda-like polypeptide 1 |
| 别名 | IGLL1, CD179 antigen-like family member B, Ig lambda 5, Ig lambda-5, IGL1 |
| 计算分子量 | 213 aa, 23 kDa |
| 观测分子量 | 20 kDa |
| GenBank蛋白编号 | BC012293 |
| 基因名称 | IGLL1 |
| Gene ID (NCBI) | 3543 |
| RRID | AB_3745454 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P15814 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD179b, also known as IGLL1, VpreB (surrogate light chain, VpreB1), is a crucial protein component of the pre-B cell receptor (pre-BCR), which is essential for the early development of B lymphocytes (B cells) in the bone marrow.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD179b antibody 87298-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
