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验证数据展示

  • WB analysis of mouse liver using 85723-4-RR (same clone as 85723-4-PBS)

    WB analysis of mouse liver using 85723-4-RR (same clone as 85723-4-PBS)

    mouse liver tissue was subjected to SDS PAGE followed by western blot with 85723-4-RR (CD164 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 85723-4-RR (same clone as 85723-4-PBS)

    IHC staining of mouse kidney using 85723-4-RR (same clone as 85723-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85723-4-RR (CD164 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

  • IF Staining of RM-1 using 85723-4-RR (same clone as 85723-4-PBS)

    IF Staining of RM-1 using 85723-4-RR (same clone as 85723-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed RM-1 cells using CD164 antibody (85723-4-RR, Clone: 242986G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85723-4-PBS in a different storage buffer formulation.

Mouse CD164 Recombinant monoclonal antibody, PBS Only

CD164 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85723-4-PBS

产品说明书

CloneNo. 242986G5

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Endolyn, MGC-24, MGC-24v, MUC-24, Multi-glycosylated core protein 24

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85723-4-PBS targets CD164 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

CatNo: Eg2645

Product name: Recombinant Mouse CD164 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 24-162 aa of NM_016898.2

Sequence: QPNITTLAPNVTEVPTTTTKVVPTTQMPTVLPETCASFNSCVSCVNATFTNNITCFWLHCQEANKTYCANEPLSNCSQVNRTDLCSVIPPTTPVPTNSTAKPTTRPSSPTPTPSVVTSAGTTNTTLTPTSQPERKSTFD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD164 antigen
别名 Endolyn, MGC-24, MGC-24v, MUC-24, Multi-glycosylated core protein 24
计算分子量21 kDa
观测分子量80 kDa
GenBank蛋白编号NM_016898.2
基因名称 Cd164
Gene ID (NCBI) 53599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9R0L9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).

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