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验证数据展示

  • IHC staining of human pancreas cancer using 12083-2-AP

    IHC staining of human pancreas cancer using 12083-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12083-2-AP (CD164 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis using 12083-2-AP

    WB analysis using 12083-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12083-2-AP (CD164 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CD164 Polyclonal antibody

CD164 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12083-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, U-87 MG cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12083-2-AP targets CD164 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2715

Product name: Recombinant human CD164 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-197 aa of BC011522

Sequence: DKNTTQHPNVTTLAPISNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIECKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQPSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFDAASFIGGIVLVLGVQAVIFFLYKFCKSKERNYHTL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD164 molecule, sialomucin
别名 CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24
计算分子量 197 aa, 21 kDa
观测分子量 82 kDa
GenBank蛋白编号BC011522
基因名称 CD164
Gene ID (NCBI) 8763
RRIDAB_2072583
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).

实验方案

Product Specific Protocols
IHC protocol for CD164 antibody 12083-2-APDownload protocol
WB protocol for CD164 antibody 12083-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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