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CD164 Polyclonal antibody

CD164 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12083-2-AP

Print datasheet

Synonyms

CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24

验证数据展示

  • FC experiment of K-562 using 12083-2-AP

    FC experiment of K-562 using 12083-2-AP

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human CD164 (12083-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

  • IHC staining of human pancreas cancer using 12083-2-AP

    IHC staining of human pancreas cancer using 12083-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12083-2-AP (CD164 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis using 12083-2-AP

    WB analysis using 12083-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12083-2-AP (CD164 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inBxPC-3 cells, U-87 MG cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12083-2-AP targets CD164 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity human
Immunogen CD164 fusion protein Ag2715 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD164 molecule, sialomucin
Synonyms CD164, CD164 molecule, sialomucin, endolyn, MUC 24, Sialomucin core protein 24
Calculated Molecular Weight 197 aa, 21 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC011522
Gene Symbol CD164
Gene ID (NCBI) 8763
RRIDAB_2072583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialomucins are a heterogeneous group of secreted or membrane-associated mucins that appear to play 2 key but opposing roles in vivo: first as cytoprotective or antiadhesive agents, and second as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor (PMID: 9680353)(PMID: 17077324). Sialomucin CD164 (MUC-24), also referred to multi-glycosylated core protein 24 (MGC24), is known to function as a receptor that regulates stem cell localization to the bone marrow. CD164 may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38(lo/-) cell proliferation. Important role of CD164 in in prostate cancer metastasis, promoting myogenesis and regulating myoblast migration so far have been revealed (PMID: 9763543)(PMID: 16859559)(PMID: 17077324).

实验方案

Product Specific Protocols
WB protocol for CD164 antibody 12083-2-APDownload protocol
IHC protocol for CD164 antibody 12083-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols