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验证数据展示

  • WB analysis using 31024-1-AP (same clone as 31024-1-PBS)

    WB analysis using 31024-1-AP (same clone as 31024-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31024-1-AP (CD163 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

    IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

    IHC staining of mouse spleen using 31024-1-AP (same clone as 31024-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31024-1-AP (CD163 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 31024-1-AP (same clone as 31024-1-PBS)

    IF Staining of mouse testis using 31024-1-AP (same clone as 31024-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CD163 antibody (31024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

    IF Staining of mouse liver using 31024-1-AP (same clone as 31024-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CD163 antibody (31024-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31024-1-PBS in a different storage buffer formulation.

Mouse CD163 Polyclonal antibody, PBS Only

CD163 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 31024-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

sCD163, M130

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31024-1-PBS targets CD163 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 mouse
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD163 antigen
别名 sCD163, M130
计算分子量121 kDa
观测分子量160 kDa
GenBank蛋白编号NM_053094.2
基因名称 Cd163
Gene ID (NCBI) 93671
RRIDAB_3669819
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2VLH6-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD163, also known as M130, is a membrane glycoprotein that belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).

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