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验证数据展示

  • WB analysis of human placenta using 83110-2-RR

    WB analysis of human placenta using 83110-2-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83110-2-RR (CD16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83110-2-RR

    IHC staining of human tonsillitis using 83110-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83110-2-RR (CD16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD16 antibody included

CD16 Recombinant monoclonal antibody

CD16 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83110-2-RR

产品说明书

CloneNo. 230337A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, Recombinant protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83110-2-RR targets CD16 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg31662

Product name: Recombinant Human FCGR3A/CD16a (F176V) protein (Myc Tag, His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: Myc & 6*His

Domain: 17-208 aa of BC017865

Sequence: GMRTEDLPKAVVFLEPQWYRVLEKDSVTLKCQGAYSPEDNSTQWFHNESLISSQASSYFIDAATVDDSGEYRCQTNLSTLSDPVQLEVHIGWLLLQAPRWVFKEEDPIHLRCHSWKNTALHKVTYLQNGKGRKYFHHNSDFYIPKATLKDSGSYFCRGLVGSKNVSSETVNITITQGLAVSTISSFFPPGYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
别名 CD16A, CD16a antigen, CD16-II, Fc gamma RIII, Fc gamma RIII alpha
计算分子量 254 aa, 29 kDa
观测分子量50 kDa
GenBank蛋白编号BC017865
基因名称 CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD16 is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B.

实验方案

Product Specific Protocols
IHC protocol for CD16 antibody 83110-2-RRDownload protocol
WB protocol for CD16 antibody 83110-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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