CD16 Polyclonal antibody
CD16 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue |
| Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | U-937 cells |
Planning an IHC experiment? We recommend our IHCeasy CD16 Ready-To-Use IHC Kit. CD16 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16559-1-AP targets CD16 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IHC, WB, ELISA |
| Cited Applications | WB, IF, FC, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CD16 fusion protein Ag9787 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Fc fragment of IgG, low affinity IIIa, receptor (CD16a) |
| Synonyms | CD16, CD16A, CD16a antigen, Fc gamma RIII, Fc gamma RIII alpha, Fc gamma RIIIa, FCG3, FCGR3, FCGR3A, FCGRIII, FCR 10, FCRIII, FCRIIIA, IGFR3, IgG Fc receptor III 2 |
| Calculated Molecular Weight | 254 aa, 29 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC017865 |
| Gene Symbol | FCGR3A |
| Gene ID (NCBI) | 2214 |
| ENSEMBL Gene ID | ENSG00000203747 |
| RRID | AB_2878279 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08637 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD16 is a 50-70-kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD16 has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD16 antibody 16559-1-AP | Download protocol |
| IHC protocol for CD16 antibody 16559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Discovery of a glucocorticoid receptor (GR) activity signature correlates with immune cell infiltration in adrenocortical carcinoma | ||
J Allergy Clin Immunol Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2. | ||
Theranostics Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring. | ||
Theranostics Non-invasive photobiomodulation treatment in an Alzheimer Disease-like transgenic rat model. | ||
J Transl Med Systematic analysis of various RNA transcripts and construction of biological regulatory networks at the post-transcriptional level for chronic obstructive pulmonary disease | ||
Int J Mol Sci C-X-C Motif Chemokine 3 Promotes the Inflammatory Response of Microglia after Escherichia coli-Induced Meningitis |
