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FITC Anti-Human CD154 (24-31)

CD154 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

FITC Fluorescent Dye

CloneNo.

24-31

Cat No : FITC-65156

Print datasheet

Synonyms

CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

验证数据展示

  • FC experiment of human peripheral blood lymphocytes using FITC-65156

    FC experiment of human peripheral blood lymphocytes using FITC-65156

    1X10^6 PMA (50 ng/ml) and ionomycin (1 ug/ml)-stimulated human PBMCs (5 hours) were surface stained with 4 ul FITC Anti-Human CD154 (FITC-65156, Clone:24-31) (green) or isotype control antibody (black). Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65156 targets CD154 in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Applications FC
Tested Reactivity Human, Non-human primates
Immunogen human CD154 fusion protein 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name CD40 ligand
Synonyms CD154, CD40 L, CD40 ligand, CD40L, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP
Calculated Molecular Weight 261 aa, 29 kDa
GenBank Accession NumberBC071754
Gene Symbol CD40LG
Gene ID (NCBI) 959
RRIDAB_2920500
Conjugate FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths498 nm / 526 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP29965
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.

实验方案

Product Specific Protocols
FC protocol for FITC CD154 antibody FITC-65156Download protocol
Standard Protocols
Click here to view our Standard Protocols