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验证数据展示

  • WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

    WB analysis using 66567-1-Ig (same clone as 66567-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66567-1-Ig (CD151 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD151 antibody included
  • IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    IHC staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66567-1-Ig (CD151 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD151 antibody included
  • IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

    IP experiment of human placenta using 66567-1-Ig (same clone as 66567-1-PBS)

    IP result of anti-CD151 (IP:66567-1-Ig, 4ug; Detection:66567-1-Ig 1:2000) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    IF Staining of human tonsillitis using 66567-1-Ig (same clone as 66567-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD151 antibody (66567-1-Ig, Clone: 2A8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66567-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50304-1

    Cytometric bead array standard curve of MP50304-1

    Cytometric bead array standard curve of MP50304-1, CD151 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66567-1-PBS. Detection antibody: 66567-2-PBS. Standard:Ag25985. Range: 0.781-50 ng/mL.

  • Featured Product
  • KD/KO验证

CD151 Monoclonal antibody, PBS Only (Capture)

CD151 Monoclonal Antibody for WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA
Cat No. 66567-1-PBS

产品说明书

CloneNo. 2A8G8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA

别名

2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD151 Ready-To-Use IHC Kit. CD151 primary antibody included.

产品信息

66567-1-PBS targets CD151 as part of a matched antibody pair:

MP50304-1: 66567-1-PBS capture and 66567-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD151 fusion protein Ag25985 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD151 molecule (Raph blood group)
别名 2A8G8, CD151 antigen, GP27, Membrane glycoprotein SFA 1, Membrane glycoprotein SFA-1
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC001374
基因名称 CD151
Gene ID (NCBI) 977
RRIDAB_2881928
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48509
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD151 (also known as TSPAN24) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members are involved in the regulation of cell development, activation, growth and motility. CD151 is broadly expressed by a variety of cell types. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. CD151 enhances cell motility, invasion and metastasis of cancer cells.