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验证数据展示

  • WB analysis using 32187-1-AP

    WB analysis using 32187-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32187-1-AP (CD147 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 32187-1-AP

    IHC staining of mouse small intestine using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 32187-1-AP

    IHC staining of mouse small intestine using 32187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 32187-1-AP (CD147 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD147 Polyclonal antibody

CD147 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32187-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen, Membrane glycoprotein gp42

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse small intestine tissue, mouse spleen tissue, mouse thymus tissue
Positive IHC detected inmouse small intestine tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32187-1-AP targets CD147 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 basigin
别名 Basic immunoglobulin superfamily, Basigin, Bsg, HT7 antigen, Membrane glycoprotein gp42
计算分子量42kDa
观测分子量35-55 kDa
GenBank蛋白编号NM_009768.2
基因名称 CD147
Gene ID (NCBI) 12215
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP18572-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 7812975). The molecule is composed of an intracellular portion, an extracellular portion, and a single transmembrane region. CD147 is expressed in various cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels (PMID: 32968061). Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte, and extracellular matrix development (PMID: 17945211). CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported thatthe spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion (Wang, Ke, et al, BioRxiv, 2020).

实验方案

Product Specific Protocols
WB protocol for CD147 antibody 32187-1-APDownload protocol
IHC protocol for CD147 antibody 32187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols