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CoraLite®594-conjugated CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4D8A9

Cat No : CL594-66153

Print datasheet

Synonyms

CD146, CD146/MCAM, MCAM, MUC18

验证数据展示

For other applications, we recommend the unconjugated version of this antibody, 66153-1-Ig

产品信息

CL594-66153 targets CD146/MCAM in applications and shows reactivity with human samples.

Tested Applications
Tested Reactivity human
Immunogen CD146/MCAM fusion protein Ag11855 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name melanoma cell adhesion molecule
Synonyms CD146, CD146/MCAM, MCAM, MUC18
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC056418
Gene Symbol MCAM
Gene ID (NCBI) 4162
RRIDAB_2883525
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43121
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.