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验证数据展示

  • WB analysis of bEnd.3 using 86159-3-RR

    WB analysis of bEnd.3 using 86159-3-RR

    bEnd.3 cells were subjected to SDS PAGE followed by western blot with 86159-3-RR (Mcam antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of bEnd.3 using 86159-3-RR

    IF Staining of bEnd.3 using 86159-3-RR

    Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using CD146/MCAM antibody (86159-3-RR, Clone: 250806F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD146/MCAM Recombinant antibody

CD146/MCAM Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86159-3-RR

产品说明书

CloneNo. 250806F12

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inbEnd.3 cells, mouse liver tissue
Positive IF/ICC detected inbEnd.3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86159-3-RR targets CD146/MCAM in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 CD146/MCAM fusion protein Eg2667 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 melanoma cell adhesion molecule
别名 CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule
计算分子量72 kDa
观测分子量120-140 kDa
GenBank蛋白编号NM_023061.2
基因名称 Mcam
Gene ID (NCBI) 84004
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8R2Y2-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

实验方案

Product Specific Protocols
WB protocol for CD146/MCAM antibody 86159-3-RRDownload protocol
IF protocol for CD146/MCAM antibody 86159-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols