验证数据展示
经过测试的应用
Positive WB detected in | bEnd.3 cells, mouse liver tissue |
Positive IF/ICC detected in | bEnd.3 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
86159-3-RR targets CD146/MCAM in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | mouse |
免疫原 | CD146/MCAM fusion protein Eg2667 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | melanoma cell adhesion molecule |
别名 | CD146, Cell surface glycoprotein MUC18, Gicerin, Mcam, Melanoma cell adhesion molecule |
计算分子量 | 72 kDa |
观测分子量 | 120-140 kDa |
GenBank蛋白编号 | NM_023061.2 |
基因名称 | Mcam |
Gene ID (NCBI) | 84004 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q8R2Y2-1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
实验方案
Product Specific Protocols | |
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WB protocol for CD146/MCAM antibody 86159-3-RR | Download protocol |
IF protocol for CD146/MCAM antibody 86159-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |