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CD146/MCAM Monoclonal antibody, PBS Only

CD146/MCAM Monoclonal Antibody for WB,IF,IHC,Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,IF,IHC,Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D8A9

Cat No : 66153-1-PBS

Print datasheet

Synonyms

CD146, MCAM, MUC18

验证数据展示

  • WB analysis of A375 using 66153-1-Ig (same clone as 66153-1-PBS)

    WB analysis of A375 using 66153-1-Ig (same clone as 66153-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66153-1-Ig (same clone as 66153-1-PBS)

    WB analysis of HepG2 using 66153-1-Ig (same clone as 66153-1-PBS)

    Untreated and PNGase F-treated lysates of HepG2 cells and L02 cells were subjected to SDS PAGE followed by western blot with 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IHC staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66153-1-Ig (CD146/MCAM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    IF Staining of human liver cancer using 66153-1-Ig (same clone as 66153-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD146/MCAM antibody (66153-1-Ig, Clone: 4D8A9 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66153-1-PBS in a different storage buffer formulation.

产品信息

66153-1-PBS targets CD146/MCAM in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,IF,IHC,Indirect ELISA
Tested Reactivity human
Immunogen CD146/MCAM fusion protein Ag11855 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name melanoma cell adhesion molecule
Synonyms CD146, MCAM, MUC18
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC056418
Gene Symbol MCAM
Gene ID (NCBI) 4162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43121
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.