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验证数据展示

  • IF Staining of RAW 264.7 using 98020-1-RR

    IF Staining of RAW 264.7 using 98020-1-RR

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of mouse peritoneal macrophages using 98020-1-RR

    FC experiment of mouse peritoneal macrophages using 98020-1-RR

    1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98020-1-RR

    Affinity and Kinetic Characterization of 98020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

Anti-Mouse CD14 Rabbit Recombinant Antibody

CD14 Uni-rAbTM Recombinant Antibody for IF/ICC, FC
Cat No. 98020-1-RR

产品说明书

CloneNo. 240225C2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

IF/ICC, FC

别名

CD14 antigen, 240225C2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inRAW 264.7 cells
Positive FC detected inmouse peritoneal macrophages

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98020-1-RR targets CD14 in IF/ICC, FC applications and shows reactivity with mouse samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD14 antigen
别名 CD14 antigen, 240225C2
计算分子量39 kDa
GenBank蛋白编号NM_009841.4
基因名称 Cd14
Gene ID (NCBI) 12475
RRIDAB_3672164
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP10810
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

实验方案

Product Specific Protocols
IF protocol for CD14 antibody 98020-1-RRDownload protocol
FC protocol for CD14 antibody 98020-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols