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  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IF Staining of human tonsillitis using 67155-1-Ig

    IF Staining of human tonsillitis using 67155-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67155-1-Ig

    IF Staining of human tonsillitis using 67155-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human spleen using 67155-1-Ig

    IHC staining of human spleen using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human appendicitis using 67155-1-Ig

    IHC staining of human appendicitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human lymphoma using 67155-1-Ig

    IHC staining of human lymphoma using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human appendicitis using 67155-1-Ig

    IHC staining of human appendicitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human ovary tumor using 67155-1-Ig

    IHC staining of human ovary tumor using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human appendicitis using 67155-1-Ig

    IHC staining of human appendicitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human tonsillitis using 67155-1-Ig

    IHC staining of human tonsillitis using 67155-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included

CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IHC, IHC-Autostainer, IF-P, ELISA
Cat No. 67155-1-Ig

产品说明书

CloneNo. 4F3A8
引用文献(7)

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, IHC-Autostainer, IF-P, ELISA and More (1)

别名

CD138, 4F3A8, SDC, SDC1, SYND1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC-Autostainer detected inhuman spleen tissue, human lymphoma tissue, human tonsillitis tissue, human appendicitis tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, human appendicitis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD138/Syndecan-1 Ready-To-Use IHC Kit. CD138/Syndecan-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)-AUTOSTAINERIHC-AUTOSTAINER : 1:10000-1:40000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67155-1-Ig targets CD138/Syndecan-1 in IHC, IHC-Autostainer, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IHC-Autostainer, IF-P, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag0823

Product name: Recombinant human SDC1,CD138 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-310 aa of BC008765

Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 syndecan 1
别名 CD138, 4F3A8, SDC, SDC1, SYND1
计算分子量 32.5 kDa
GenBank蛋白编号BC008765
基因名称 CD138
Gene ID (NCBI) 6382
RRIDAB_2882452
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18827
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

实验方案

Product Specific Protocols
IF protocol for CD138/Syndecan-1 antibody 67155-1-IgDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 67155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
View Article
humanIF

Nat Commun

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection

Authors - Pei-Fen Zhang
View Article
humanIF

Adv Sci (Weinh)

Targeting of the CD161 Inhibitory Receptor Enhances Bone-Marrow-Resident Memory CD8(+) T-Cell-Mediated Immunity against Multiple Myeloma.

Authors - Liwen Wang
View Article
humanIF

Dev Cell

SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements

Authors - Yanglin Chen
View Article
humanIHC

Exp Cell Res

ZFP36 and CEBPA are macrophage-associated prognostic biomarkers linked to glomerular endothelial inflammation in ANCA-associated glomerulonephritis.

Authors - Anqi Ni
View Article
humanIF

Placenta

HIF-2α / HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia.

Authors - Qianghua Wang
View Article
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