验证数据展示

IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IF Staining of human tonsillitis using 67155-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human tonsillitis using 67155-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD138/Syndecan-1 antibody (67155-1-Ig, Clone: 4F3A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IHC staining of human appendicitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human lymphoma using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human appendicitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human ovary tumor using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human appendicitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
IHC staining of human tonsillitis using 67155-1-Ig
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67155-1-Ig (CD138/Syndecan-1 antibody) at dilution of 1:20000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included

CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IHC, IF-P, ELISA

Cat No. 67155-1-Ig

产品说明书

CloneNo. 4F3A8

引用文献(7)

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IHC, IF-P, ELISA and More (1)

别名

CD138, 4F3A8, SDC, SDC1, SYND1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (7)
实验方案

产品说明书

MSDS

经过测试的应用

Positive IHC detected in	human tonsillitis tissue, human lymphoma tissue, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human tonsillitis tissue

Planning an IHC experiment? We recommend our IHCeasy CD138/Syndecan-1 Ready-To-Use IHC Kit. CD138/Syndecan-1 primary antibody included.

应用	推荐稀释比
Immunohistochemistry (IHC)	IHC : 1:4000-1:16000
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHC	See 1 publications below
IF	See 6 publications below

产品信息

67155-1-Ig targets CD138/Syndecan-1 in IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用	IHC, IF-P, ELISA Application Description
文献引用应用	IHC, IF
经测试反应性	human
文献引用反应性	human
免疫原	CatNo: Ag0823 Product name: Recombinant human SDC1,CD138 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-310 aa of BC008765 Sequence: MRRAALWLWLCALALSLQPALPQIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHQASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKEVLGGVIAVGLVGLIFAVCLVGFMLYRMKKKDEGSYSLEEPKQANGGAYQKPTKQEEFYA 种属同源性预测
宿主/亚型	Mouse / IgG2a
抗体类别	Monoclonal
产品类型	Antibody
全称	syndecan 1
别名	CD138, 4F3A8, SDC, SDC1, SYND1
计算分子量	32.5 kDa
GenBank蛋白编号	BC008765
基因名称	CD138
Gene ID (NCBI)	6382
RRID	AB_2882452
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
UNIPROT ID	P18827
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Syndecan-1 (SDC1), also named CD138, is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. SDC1 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

实验方案

Product Specific Protocols
IF protocol for CD138/Syndecan-1 antibody 67155-1-Ig	Download protocol
IHC protocol for CD138/Syndecan-1 antibody 67155-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	IF	Nat Commun Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection Authors - Pei-Fen Zhang View Article
human	IF	Dev Cell SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements Authors - Yanglin Chen View Article
human	IF	Transl Cancer Res By integrating single-cell RNA sequencing and bulk RNA sequencing, plasma cells signature and tertiary lymphoid structures were verified to contribute to outcome in lung adenocarcinoma Authors - Zetian Gong View Article
human	IF	Adv Sci (Weinh) Targeting of the CD161 Inhibitory Receptor Enhances Bone-Marrow-Resident Memory CD8(+) T-Cell-Mediated Immunity against Multiple Myeloma. Authors - Liwen Wang View Article
human	IF	Placenta HIF-2α / HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia. Authors - Qianghua Wang View Article

验证数据展示

IHC staining of human tonsillitis using 67155-1-Ig

IHC staining of human tonsillitis using 67155-1-Ig

IF Staining of human tonsillitis using 67155-1-Ig

IF Staining of human tonsillitis using 67155-1-Ig

IHC staining of human appendicitis using 67155-1-Ig

IHC staining of human tonsillitis using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human lymphoma using 67155-1-Ig

IHC staining of human tonsillitis using 67155-1-Ig

IHC staining of human appendicitis using 67155-1-Ig

IHC staining of human ovary tumor using 67155-1-Ig

IHC staining of human appendicitis using 67155-1-Ig

IHC staining of human tonsillitis using 67155-1-Ig

IHC staining of human tonsillitis using 67155-1-Ig

CD138/Syndecan-1 Monoclonal antibody

CD138/Syndecan-1 Monoclonal Antibody for IHC, IF-P, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Establishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection

SP6 controls human cytotrophoblast fate decisions and trophoblast stem cell establishment by targeting MSX2 regulatory elements

By integrating single-cell RNA sequencing and bulk RNA sequencing, plasma cells signature and tertiary lymphoid structures were verified to contribute to outcome in lung adenocarcinoma

Targeting of the CD161 Inhibitory Receptor Enhances Bone-Marrow-Resident Memory CD8(+) T-Cell-Mediated Immunity against Multiple Myeloma.

HIF-2α / HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia.

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