CoraLite® Plus 750 Anti-Human CD13 (WM15)
CD13 Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human, Non-human primates
Applications
FC
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
WM15
验证数据展示
经过测试的应用
| Positive FC detected in | human blood |
For other applications, we recommend the unconjugated version of this antibody, 65186-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-65186 targets CD13 in FC applications and shows reactivity with Human, Non-human primates samples.
| Tested Applications | FC |
| Tested Reactivity | Human, Non-human primates |
| Immunogen | Human AML cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | alanyl (membrane) aminopeptidase |
| Synonyms | Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN |
| Calculated Molecular Weight | 110 kDa |
| GenBank Accession Number | BC058928 |
| Gene Symbol | ANPEP |
| Gene ID (NCBI) | 290 |
| ENSEMBL Gene ID | ENSG00000166825 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | P15144 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD13, also named as APN, ANPEP (aminopeptidase N) or PEPN, is belongs to the peptidase M1 family. CD13 is a heavily glycosylated, ~150-240 kDa, type-II membrane, expressed by most cells of myeloid origin including monocytes, macrophages, granulocytes, and their hematopoietic precursors. It is also abundantly expressed in the brush border of epithelial cells from renal proximal tubules and small intestine, in prostatic epithelial cells, in bile duct canaliculi, in mast cells, and, in some cases, in fibroblasts and smooth muscle cells. CD13 is a multifunctional protein and plays varying roles in cell migration, cell proliferation, cell differentiation and so on. CD13 participates in angiogenesis generating and modulating angiogenic signals, and can be a marker of angiogenic vessels. CD13 is also a pan-myeloid marker, present on mature granulocytes and monocytes. (PMID: 8805662, 10098327, 18603472, 18097955, 17897790, 17888402, 21339174)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD13 antibody CL750-65186 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
