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CoraLite®594 Anti-Mouse CD11b (M1/70)

CD11b Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse, Human

Applications

FC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

M1/70

Cat No : CL594-65055

Print datasheet

Synonyms

Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

验证数据展示

  • FC experiment of mouse bone marrow cells using CL594-65055

    FC experiment of mouse bone marrow cells using CL594-65055

    1X10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite®594 Anti-Mouse CD11b (CL594-65055, Clone:M1/70) or and 0.25 ug CoraLite®594 Rat IgG2b Isotype Control (LTF-2) (CL594-65211, Clone: LTF-2), and 0.25 ug APC Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (APC-65140, Clone: RB6-8C5). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65055-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65055 targets CD11b in FC applications and shows reactivity with Mouse, Human samples.

Tested Applications FC
Tested Reactivity Mouse, Human
Immunogen C57BL/10 mouse splenic T cells and concanavalin A-activated C57BL/10 splenocytes 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name integrin alpha M
Synonyms Cd11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1
GenBank Accession NumberBC156991
Gene Symbol Itgam
Gene ID (NCBI) 16409
RRIDAB_2919927
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDE9Q604
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

实验方案

Product Specific Protocols
FC protocol for CL594 CD11b antibody CL594-65055Download protocol
Standard Protocols
Click here to view our Standard Protocols